Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast tissue showing cytoplasmic and nuclear staining of glandular cells.Application
Western blot analysis of Stat5a expression in non-transfected: (A) and human Stat5a transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Stat5a expression in non-transfected: (A) and human Stat5a transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Stat5a expression in non-transfected 293T: (A), human Stat5a transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear localization.Application
Western blot analysis of Stat5 expression in K-562 nuclear extract (A) and K-562 whole cell lysate (B).| Product Name | Stat5 Antibody (H-134) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 661-794 mapping at the C-terminus of Stat5a of human origin |
| Modification Notes | Signal transducer and activator of transcription 5a (Stat5a) and Stat5b, which share 96% homology, undergo receptor tyrosine kinase or G protein-coupled receptor-dependent phosphorylation in response to cytokines or growth factors, and then form homo- or heterodimers that translocate to the nucleus, where they initiate transcription. Activation of Stat5a via IL-2, IL-3, IL-7 GM-CSF, erythropoietin, thrombopoietin and growth hormones influences proliferation, differentiation and apoptosis in lymphohematopoietic cells. Phosphorylation of Stat5a at Ser 127/Ser 128 and Ser 779 are contigent on ErbB4-mediated activation of Stat5a. Activation of Stat5b via IL-2, IL-4, CSF1 and growth hormones influences TCR signaling, apoptosis, adult mammary gland development and sexual dimorphism of liver gene expression. Stat5b is the major liver-expressed Stat5 form that has been shown to fuse with the retinoic acid receptor a gene in acute promyelocytic leukemias (APLL). Stat5a/B null mice have severely impaired lymphoid development and differentiation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of Stat5a and Stat5b of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 661-794 mapping at the C-terminus of Stat5a of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 661-794 mapping at the C-terminus of Stat5a of human origin |
| Entrez Gene | 6776 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast tissue showing cytoplasmic and nuclear staining of glandular cells.
Application
Western blot analysis of Stat5a expression in non-transfected: (A) and human Stat5a transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Stat5a expression in non-transfected: (A) and human Stat5a transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Stat5a expression in non-transfected 293T: (A), human Stat5a transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear localization.
Application
Western blot analysis of Stat5 expression in K-562 nuclear extract (A) and K-562 whole cell lysate (B).| Application Notes | recommended for detection of Stat5a and Stat5b of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
