Application
Western blot analysis of TBL1X expression in non-transfected: (A) and human TBL1X transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing nuclear and cytoplasmic staining of glandular cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse testis tissue showing cytoskeletal localization.Application
Immunofluorescence staining of methanol-fixed MCF7 cells showing cytoskeletal localization.Application
Western blot analysis of mouse recombinant TBL1 fusion protein.| Product Name | TBL1 Antibody (H-367) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 211-577 of TBLβ1X of human origin |
| Modification Notes | TBL1, for transducin ∫-like 1, is a ubiquitously expressed protein that contains six distinct ∫-transducin repeats, known also as WD40 repeats, within the C-terminal domain. Transducin ∫-like 1 Y-linked protein (TBL1Y), also designated F-box-like/WD-repeat protein, and transducin ∫-like 1 X protein (TBL1X), also known as SMAP55, are nuclear F-box-like proteins. They are important in the ubiquitin/19S proteasome complex recruitment to nuclear receptor-regulated transcription units. TBL1X is a part of the N-CoR repressor complex together with N-CoR1, N-CoR2, HDAC3, TBL1R, CORO2A and GPS2. It is also a component of the E3 ubiquitin ligase complex. TBL1X, which can interact with Histones H2B, H3a and H4, is similar to TBL1Y but is localized on chromosome Xp22.3. Defects in TBL1X may cause an X-linked human disorder called ocular albinism with late-onset sensorineural deafness (OASD). TBL1Y is an X-degenerate gene that is homologous to TBL1X. TBL1Y, a single-copy gene, localizes to human chromosome Yp11.2 in the male-specific region of chromosome Y (MSY). This region of the Y chromosome does not engage in X-Y crossover events. TBL1Y is primarily expressed in fetal brain and prostate. TBL1X and TBL1Y are crucial in nuclear receptor mediated transcription activation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAChIP |
| recommended for detection of TBL1X and TBL1Y of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 211-577 of TBL&beta 1X of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 211-577 of TBLβ1X of human origin |
| Entrez Gene | 6907 |
|---|
Application
Western blot analysis of TBL1X expression in non-transfected: (A) and human TBL1X transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing nuclear and cytoplasmic staining of glandular cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse testis tissue showing cytoskeletal localization.
Application
Immunofluorescence staining of methanol-fixed MCF7 cells showing cytoskeletal localization.
Application
Western blot analysis of mouse recombinant TBL1 fusion protein.| Application Notes | recommended for detection of TBL1X and TBL1Y of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
