Application
Western blot analysis of AQP5 expression in non-transfected: (A) and human AQP5 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and cytoplasmic localization.Application
Western blot analysis of AQP5 expression in KNRK whole cell lysate.| Product Name | AQP5 Antibody (H-200) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 66-265 mapping at the C-terminus of AQP5 of human origin |
| Modification Notes | Aquaporins (AQPs) are a large family of integral membrane water transport channel proteins that facilitate the transport of water through the cell membrane. This function is conserved in animals, plants and bacteria. Many isoforms of aquaporin have been identified in mammals, designated AQP0 through AQP10. Aquaporins are widely distributed and it is not uncommon for more than one type of AQP to be present in the same cell. Although most aquaporins are only permeable to water, AQP3, AQP7, AQP9 and one of the two AQP10 transcripts are also permeable to urea and glycerol. AQP2 is the only water channel that is activated by vasopressin to enhance water reabsorption in the kidney collecting duct. Aquaporins are involved in renal water absorption, generation of pulmonary secretions, lacrimation, and the secretion and reabsorption of cerebrospinal fluid and aqueous humor. In the lung, AQP5 is responsible for the majority of water transport across the apical membrane of type I alveolar epithelial cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of AQP5, and, to a lesser extent, AQP0, 2, 4 and 6 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 66-265 mapping at the C-terminus of AQP5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 66-265 mapping at the C-terminus of AQP5 of human origin |
| Entrez Gene | 362 |
|---|
Application
Western blot analysis of AQP5 expression in non-transfected: (A) and human AQP5 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and cytoplasmic localization.
Application
Western blot analysis of AQP5 expression in KNRK whole cell lysate.| Application Notes | recommended for detection of AQP5, and, to a lesser extent, AQP0, 2, 4 and 6 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
