Application
Western blot analysis of BUB3 expression in non-transfected: (A) and mouse BUB3 transfected: (B) 293T whole cell lysates and mouse embryo tissue extract (C).Application
Western blot analysis of BUB3 expression in Jurkat nuclear extract.| Product Name | BUB3 Antibody (H-100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 229-328 mapping at the C-terminus of BUB3 of human origin |
| Modification Notes | BUB3 (budding uninhibited by benzimidazoles 3 homolog), also known as BUB3L or hBUB3, is a conserved component of the mitotic spindle assembly complex (MCC). It contains five WD repeat domains and forms cell cycle constitutive complexes with BUB1 and BUBR1. BUB3 is essential for the kinetochore localization of BUB1 and BUBR1. As a component of the MCC, BUB3 is involved in the essential spindle checkpoint pathway that operates during early embryogenesis. The spindle checkpoint pathway functions to postpone the initiation of anaphase until chromosomes are properly attached to the spindle. This acts to ensure accurate chromosome segregation. In addition, BUB3 plays a role in regulating the establishment of correct kinetochore-microtubule attachments. BUB3 is also thought to bind Tctex1L (or DYNLT3), a dynein light chain. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of BUB3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 229-328 mapping at the C-terminus of BUB3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 229-328 mapping at the C-terminus of BUB3 of human origin |
| Entrez Gene | 9184 |
|---|
Application
Western blot analysis of BUB3 expression in non-transfected: (A) and mouse BUB3 transfected: (B) 293T whole cell lysates and mouse embryo tissue extract (C).
Application
Western blot analysis of BUB3 expression in Jurkat nuclear extract.| Application Notes | recommended for detection of BUB3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
