Application
Western blot analysis of claudin-19 expression in non-transfected: (A) and human claudin-19 transfected: (B) 293T whole cell lysates.| Product Name | claudin-19 Antibody (H-65) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 160-224 mapping at the C-terminus of claudin-19 of human origin |
| Modification Notes | The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the claudins, Occludin and Junction adhesion molecules. Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is often highly restricted to specfic regions of different tissues and may have an important role in transcellular transport through tight junctions. Claudin-19 is a 224 amino acid multi-pass membrane protein that belongs to the claudin family and is expressed as two isoforms due to alternative splicing events. Defects in the gene encoding claudin-19 are the cause of hypomagnesemia renal with ocular involvement (HOMGO), a renal disease characterized by hypomagnesemia, hypercalciuria and nephrocalcinosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of claudin-19 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 160-224 mapping at the C-terminus of claudin-19 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 160-224 mapping at the C-terminus of claudin-19 of human origin |
Application
Western blot analysis of claudin-19 expression in non-transfected: (A) and human claudin-19 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of claudin-19 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
