Application
Western blot analysis of DCTD expression in non-transfected 293: (A), human DCTD transfected 293: (B), K-562 (C) and WI 38 (D) whole cell lysates.| Product Name | DCTD Antibody (FL-178) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-178 representing full length DCTD of human origin |
| Modification Notes | DCTD (Deoxycytidylate deaminase), also known as dCMP deaminase, is a 178 amino acid allosteric enzyme that exists as a homohexamer and belongs to the cytidine and deoxycytidylate deaminase protein family. Using zinc as a cofactor, DCTD catalyzes the deamination of dCMP to dUMP, thereby producing the nucleotide substrate (dUMP) that is used by thymidylate synthase (TS). TS uses 5,10-methyl-enetetrahydrofolate (methylene-THF) and dUMP in the synthesis of 2'-deoxythymidine-5'-monophosphate (dTMP), an essential precursor for DNA biosynthesis. Due to its role in the synthesis of dUMP, DCTC plays an important role in the creation of DNA. The activity of DCTD is regulated by the presence of dCTP and dTTP, two end products in the DCTD metabolic pathway. Multiple isoforms of DCTD are expressed due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DCTD of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-178 representing full length DCTD of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-178 representing full length DCTD of human origin |
| Entrez Gene | 1635 |
|---|
Application
Western blot analysis of DCTD expression in non-transfected 293: (A), human DCTD transfected 293: (B), K-562 (C) and WI 38 (D) whole cell lysates.| Application Notes | recommended for detection of DCTD of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
