Application
Western blot analysis of human DNA-PKCS expression in DNA-PKCS shRNA Plasmid transfected: (A), control shRNA Plasmid transfected: (B) and non-transfected (C) 293T cells. Blot probed with DNA-PKCS (H-163): . Filamin 1 (E-3): used as specificity and loading control.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing nuclear staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of DNA-PKCS expression in RAW 264.7 (A), M1 (B) and WR19L (C) whole cell lysates.Application
Western blot analysis of DNA-PKCS expression in HeLa (A), MOLT-4 (B) and K-562 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor (A) and immunofluorescence staining of methanol-fixed HeLa cells (B) showing nuclear localization.| Product Name | DNA-PKCS Antibody (H-163) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 3965-4127 mapping at the C-terminus of DNA-PKCS (DNA-dependent protein kinase catalytic subunit) of human origin |
| Modification Notes | The phosphatidylinositol kinase (PIK) family members fall into two distinct subgroups. The first subgroup contains proteins such as the PI 3- and PI 4-kinases and the second group comprises the PIK-related kinases. The PIK-related kinases include Atm, DNA-PKCS and FRAP. These proteins have in common a region of homology at their carboxy termini that is not present in the PI 3- and PI 4-kinases. The Atm gene is mutated in the autosomal recessive disorder ataxia telangiectasia (AT) that is characterized by cerebellar degeneration (ataxia) and the appearance of dilated blood vessels (telangiectases) in the conjunctivae of the eyes. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and they display delays in p53 induction. DNA-PK is a heterotrimeric DNA binding enzyme that is composed of a large subunit, DNA-PKCS, and two smaller subunits collectively known as Ku. The loss of DNA-PK leads to defects in DSB repair and V(D)J recombination. FRAP can autophosphorylate on serine and bind to rapamycin/FKBP. FRAP is also an upstream regulator of S6 kinase and has been implicated in the regulation of p27 and p21 expression. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of DNA-PKCS of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 3965-4127 mapping at the C-terminus of DNA-PKCS (DNA-dependent protein kinase catalytic subunit) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 3965-4127 mapping at the C-terminus of DNA-PKCS (DNA-dependent protein kinase catalytic subunit) of human origin |
| Entrez Gene | 5591 |
|---|
Application
Western blot analysis of human DNA-PKCS expression in DNA-PKCS shRNA Plasmid transfected: (A), control shRNA Plasmid transfected: (B) and non-transfected (C) 293T cells. Blot probed with DNA-PKCS (H-163): . Filamin 1 (E-3): used as specificity and loading control.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing nuclear staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of DNA-PKCS expression in RAW 264.7 (A), M1 (B) and WR19L (C) whole cell lysates.
Application
Western blot analysis of DNA-PKCS expression in HeLa (A), MOLT-4 (B) and K-562 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor (A) and immunofluorescence staining of methanol-fixed HeLa cells (B) showing nuclear localization.| Application Notes | recommended for detection of DNA-PKCS of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
