Application
Western blot analysis of CUL-4A expression in non-transfected (A) and human CUL-4A transfected (B) 293 whole cell lysates.| Product Name | CUL-4A Antibody (P-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of CUL-4A of human origin by WB and ELISA |
| Modification Notes | CUL-4A is a member of the cullin family of proteins that is involved in the ubiquitin-mediated degradation of cell cycle regulators. CUL-4A regulates cell cycle progression during differentiation, and overexpression of this protein significantly increases the number of cells in S phase and reduces the number that accumulate in G0/G1 phase. CUL-4A localizes to the cytoplasm where it stimulates ubiquitylation and degradation of the HoxA9 homeodomain protein, a key regulator of hematopoiesis and embryonic development. CUL-4A also stimulates the degradation of the damaged DNA-binding protein (DDB) that plays a role in DNA repair and is involved in the repair deficiency disease xeroderma pigmentosum. The CUL-4A gene is amplified and overexpressed in breast cancer, implicating the protein in tumorigenesis and/or tumor progression. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of CUL-4A of human origin by WB and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of CUL-4A of human origin by WB and ELISA |
| Entrez Gene | 8451 |
|---|
Application
Western blot analysis of CUL-4A expression in non-transfected (A) and human CUL-4A transfected (B) 293 whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
