Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of Mcl-1 expression in non-transfected 293T: (A), human Mcl-1 transfected 293T: (B) and Ramos (C) whole cell lysates.Application
Western blot analysis of Mcl-1 expression in BJAB whole cell lysate.Application
Western blot analysis of Mcl-1 expression in non-transfected 293T: (A), mouse Mcl-1 transfected 293T: (B) and BJAB (C) whole cell lysates.Application
Western blot analysis of Mcl-1 long and short expression in BJAB whole cell lysate.| Product Name | Mcl-1 Antibody (H-260) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-260 mapping at the N-terminus of Mcl-1 of human origin |
| Modification Notes | B-cell CLL/lymphoma 2 (Bcl-2) blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Myeloid cell leukemia 1 (Mcl-1) shares sequence homology with Bcl-2 and further resembles Bcl-2 in that its expression promotes cell viability. p53 and Mcl-1 demonstrate opposing effects on mitochondrial apoptosis by mediating Bcl-2 antagonist killer (Bak) activity. Mcl-1 is an important and specific regulator that is necessary for the homeostasis of early hematopoietic progenitors. Glycogen synthase kinase 3 (GSK3) controls Mcl-1 stability, which has an effect on the regulation of apoptosis by growth factors, PI 3-kinase and AKT. Mice with a deficiency of the Mcl-1 protein show a significant reduction in B and T lymphocytes similar to the effects observed in IL-7- or IL-7R-deficient mice. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Mcl-1 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-260 mapping at the N-terminus of Mcl-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-260 mapping at the N-terminus of Mcl-1 of human origin |
| Entrez Gene | 4170 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of Mcl-1 expression in non-transfected 293T: (A), human Mcl-1 transfected 293T: (B) and Ramos (C) whole cell lysates.
Application
Western blot analysis of Mcl-1 expression in BJAB whole cell lysate.
Application
Western blot analysis of Mcl-1 expression in non-transfected 293T: (A), mouse Mcl-1 transfected 293T: (B) and BJAB (C) whole cell lysates.
Application
Western blot analysis of Mcl-1 long and short expression in BJAB whole cell lysate.| Application Notes | recommended for detection of Mcl-1 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
