Application
Western blot analysis of Cdc2 p34 expression in untreated (A) and Licochalcone-A, Synthetic treated (B) PC-3 whole cell lysates. Note down regulation of Cdc2 p34 expression in lane B.Application
Western blot analysis of ALS2CR7 expression in non-transfected 293: (A), human ALS2CR7 transfected 293: (B) and HeLa (C) whole cell lysates.Application
Antibodies tested include p-Cdc2 p34 (Thr 161)-R: (A,B,C) and p-Cdc2 p34 (PSTAIRE): (D,E,F).Application
Antibodies tested include p-Cdc2 p34 (Tyr 15)-R: (A-E) and p-Cdc2 p34 (PSTAIRE): (F-J).Application
Western blot analysis of Cdk2 expression in non-transfected 293T: (A), human Cdk2 transfected 293T: (B) and NAMALWA (C) whole cell lysates.Application
Western blot analysis of Cdc2 p34 expression in non-transfected 293T: (A), mouse Cdc2 p34 transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of Cdk2 expression in non-transfected: (A) and mouse Cdk2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Cdc2 p34 expression in non-transfected 293: (A), human Cdc2 p34 transfected 293: (B) and HeLa (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of Cdc2 p34 expression in HeLa nuclear extract (A) and MCF7 whole cell lysate (B).| Product Name | Cdc2 p34 Antibody (PSTAIRE) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within the conserved PSTAIRE domain of Cdc2 p34 of human origin |
| Modification Notes | In vertebrates, as in yeast, multiple cyclins have been identified, including a total of eight such regulatory proteins in mammals. In contrast to the situation in yeast, the Cdc2 p34 kinase is not the only catalytic subunit identified in vertebrates that can interact with cyclins. While Cdc2 p34 is essential for the G2 to M transition in vertebrate cells, a second Cdc2-related kinase has also been implicated in cell cycle control. This protein, designated cyclin-dependent kinase 2 (Cdk2) p33, also binds to cyclins and its kinase activity is temporally regulated during the cell cycle. Several additional Cdc2 p34-related cyclin dependent kinases have been identified. These include Cdk3-Cdk8, PCTAIRE-1-3 and KKIALRE. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Cdc2 p34 and other cyclin dependent kinases containing PSTAIRE or PFTAIRE motifs of mouse, rat, human, avian, yeast, Drosophila and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within the conserved PSTAIRE domain of Cdc2 p34 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within the conserved PSTAIRE domain of Cdc2 p34 of human origin |
| Entrez Gene | 983 |
|---|
Application
Western blot analysis of Cdc2 p34 expression in untreated (A) and Licochalcone-A, Synthetic treated (B) PC-3 whole cell lysates. Note down regulation of Cdc2 p34 expression in lane B.
Application
Western blot analysis of ALS2CR7 expression in non-transfected 293: (A), human ALS2CR7 transfected 293: (B) and HeLa (C) whole cell lysates.
Application
Antibodies tested include p-Cdc2 p34 (Thr 161)-R: (A,B,C) and p-Cdc2 p34 (PSTAIRE): (D,E,F).
Application
Antibodies tested include p-Cdc2 p34 (Tyr 15)-R: (A-E) and p-Cdc2 p34 (PSTAIRE): (F-J).
Application
Western blot analysis of Cdk2 expression in non-transfected 293T: (A), human Cdk2 transfected 293T: (B) and NAMALWA (C) whole cell lysates.
Application
Western blot analysis of Cdc2 p34 expression in non-transfected 293T: (A), mouse Cdc2 p34 transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of Cdk2 expression in non-transfected: (A) and mouse Cdk2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Cdc2 p34 expression in non-transfected 293: (A), human Cdc2 p34 transfected 293: (B) and HeLa (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of Cdc2 p34 expression in HeLa nuclear extract (A) and MCF7 whole cell lysate (B).| Application Notes | recommended for detection of Cdc2 p34 and other cyclin dependent kinases containing PSTAIRE or PFTAIRE motifs of mouse, rat, human, avian, yeast, Drosophila and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
