Application
Western blot analysis of GRAIL expression in non-transfected 293T: (A), human GRAIL transfected 293T: (B) and Hep G2 (C) whole cell lysates.Application
Western blot analysis of GRAIL expression in non-transfected 293T: (A), human GRAIL transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of GRAIL expression in Hep G2 whole cell lysate.| Product Name | GRAIL Antibody (M-17) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against GRAIL of human origin |
| Modification Notes | GRAIL, also known as ring finger protein 128, is a 428 amino acid type I transmembrane protein localized to the intracytoplasmic membrane. GRAIL contains a protease-associated (PA) domain and a RING finger domain, which binds to E2 ubiquitin-conjugating enzymes. When under anergic conditions, GRAIL functions as an E3 ubiquitin-protein ligase that inhibits IL-2, IL-4 and various other cytokines. GRAIL is also thought to be involved in the patterning of the dorsal ectoderm during development. Expressed in an asymmetric perinuclear punctate manner, GRAIL colocalizes with Rab 7, GRP 78 and Syntaxin 5. GRAIL is expressed as 2 isoforms produced by alternative splicing. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| recommended for detection of GRAIL of human origin by WB, IP and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against GRAIL of human origin |
|---|---|
| Tissue Specificity | raised against GRAIL of human origin |
| Entrez Gene | 79589 |
|---|
Application
Western blot analysis of GRAIL expression in non-transfected 293T: (A), human GRAIL transfected 293T: (B) and Hep G2 (C) whole cell lysates.
Application
Western blot analysis of GRAIL expression in non-transfected 293T: (A), human GRAIL transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of GRAIL expression in Hep G2 whole cell lysate.| Application Notes | recommended for detection of GRAIL of human origin by WB, IP and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
