Application
Western blot analysis of DNA pol δ cat expression in K-562 (A) and MOLT-4 (B) nuclear extracts.Application
Western blot analysis of DNA pol δ cat expression in NIH/3T3 (A), KNRK (B) and RAW 264.7 (C) nuclear extracts.Application
Immunoperoxidase staining of methanol-fixed Jurkat cells showing nuclear staining.| Product Name | DNA pol δ cat Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 of DNA pol δ catalytic chain of human origin |
| Modification Notes | DNA replication, recombination and repair, all of which are necessary for genome stability, require the presence of exonucleases (1). In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches (2). Exonucleases involved in these processes include DNA polymerases, including DNA pol ∂ and é. DNA pol ∂ consists of two subunits, p125 which interacts directly with the sliding DNA clamp protein PCNA, and p50 (3,4). DNA pol ∂ can be regulated by cell cycle proteins (5). DNA pol é is a multiple subunit enzyme, the catalytic subunit of which is encoded by the POL2 gene (6,7). The exact reactions catalyzed by DNA pol ∂ and é on leading and lagging strands have not yet been elucidated. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of DNA pol δ catalytic chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 of DNA pol &delta catalytic chain of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 of DNA pol δ catalytic chain of human origin |
| Entrez Gene | 5424 |
|---|
Application
Western blot analysis of DNA pol δ cat expression in K-562 (A) and MOLT-4 (B) nuclear extracts.
Application
Western blot analysis of DNA pol δ cat expression in NIH/3T3 (A), KNRK (B) and RAW 264.7 (C) nuclear extracts.
Application
Immunoperoxidase staining of methanol-fixed Jurkat cells showing nuclear staining.| Application Notes | recommended for detection of DNA pol δ catalytic chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
