Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear localization.Application
Western blot analysis of CHD9 expression in LADMAC whole cell lysate.Application
Western blot analysis of CHD9 expression in non-transfected: (A) and human CHD9 transfected: (B) 293T whole cell lysates.| Product Name | CHD9 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 225-514 mapping near the N-terminus of CHD9 of human origin |
| Modification Notes | CHD9 (chromodomain-helicase-DNA-binding protein 9), also known as chromatin-related mesenchymal modulator (CReMM), PPAR-alpha-interacting complex protein 320 kDa, kismet homolog 2 or CHROM1, is a 2,897 amino acid protein belonging to the Snf2/Rad54 helicase family. The CHD family of proteins are ATP-dependent chromatin remodeling enzymes which combine chromodomains with SWI2/Snf2 ATPase/helicase motifs and DNA-binding capability. Localized to the cytoplasm and the nucleus, CHD9 contains two chromodomains, one ATP-binding helicase domain and one C-terminal helicase domain. Chromodomains are protein regions of about 40-50 amino acid residues found in proteins associated with chromatin remodeling and manipulation. The domain is highly conserved among both plants and animals and is found in a large variety of proteins from many genomes. CHD9 acts as a transcriptional coactivator for PPARå and may also be an ATP-dependent chromatin remodeling protein. CHD9 is widely expressed at low levels and is present as three isoforms produced by alternative splicing. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CHD9 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 225-514 mapping near the N-terminus of CHD9 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 225-514 mapping near the N-terminus of CHD9 of human origin |
| Entrez Gene | 80205 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear localization.
Application
Western blot analysis of CHD9 expression in LADMAC whole cell lysate.
Application
Western blot analysis of CHD9 expression in non-transfected: (A) and human CHD9 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of CHD9 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
