Application
Western blot analysis of TBC1D3C expression in non-transfected: (A) and human TBC1D3C transfected: (B) 293T whole cell lysates.| Product Name | TBC1D3/3B/3C/3G Antibody (H-54) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 496-549 mapping at the C-terminus of TBC1D3 of human origin |
| Modification Notes | The TBC1D3 proteins, some of which include TBC1D3, TBC1D3B, TBC1D3C and TBC1D3G, contain Rab-GAP TBC domains and are thought to function as GTPase activating proteins for Rab 5, thereby mediating Rab 5 function throughout the cell. TBC1D3 family members exhibit different expression patterns and may be involved in the pathogenesis of a variety of carcinomas, including prostate cancer. The gene encoding TBC1D3, TBC1D3B, TBC1D3C and TBC1D3G maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and Li-Fraumeni syndrome. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of TBC1D3, TBC1D3B, TBC1D3C and TBC1D3G of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 496-549 mapping at the C-terminus of TBC1D3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 496-549 mapping at the C-terminus of TBC1D3 of human origin |
| Entrez Gene | 84218 |
|---|
Application
Western blot analysis of TBC1D3C expression in non-transfected: (A) and human TBC1D3C transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of TBC1D3, TBC1D3B, TBC1D3C and TBC1D3G of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
