| Product Name | S-probe Antibody (K-14) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within the S-Tag encoded-domain of pET-29a-c+ expression vector |
| Modification Notes | Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification or quantification of the target protein (1-4). A novel system that addresses the issue of protein quantification utilizes an enzymatically cleaved derivative of bovine RNase A, called RNase S (5,6). RNase S is composed of the S-peptide (residues 1-20) and the S-protein (residues 21-124), which together form the active enzyme (6). Although the S-protein itself is inactive, addition of the S-peptide, or a fusion protein tagged with the S-peptide, will reconstitute its enzymatic activity (6) which can then be assayed on a poly(C) substrate and quantified by spectroscopy. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBELISA |
| recommended for detection of pET-29a-c+ encoded fusion proteins by WB and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within the S-Tag encoded-domain of pET-29a-c+ expression vector |
|---|---|
| Tissue Specificity | epitope mapping within the S-Tag encoded-domain of pET-29a-c+ expression vector |
| Application Notes | recommended for detection of pET-29a-c+ encoded fusion proteins by WB and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
