IHC
The immunostaining of the 3 melatonin nuclear receptors RORα (A, D), RORβ (B, E) and RORγ (C, F) in the spleen of broilers at 14 D.| Product Name | RORβ Antibody (H-42) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Nuclear receptors that lack a defined ligand are classified as orphan nuclear receptors. Retinoic acid receptor-related orphan nuclear receptor (ROR) proteins RORå, ROR∫ and ROR© are members of the nuclear hormone receptor superfamily. Unlike other members of the nuclear hormone receptor superfamily that bind DNA as homo- or heterodimers, ROR proteins directly bind the hormone response element (HRE) DNA sequence as monomers. RORå has multiple isoforms that share common DNA- and putative ligand-binding domains, but differ in their amino-terminal domains, which are generated by alternative RNA processing. ROR∫ is primarily expressed in brain and other areas of the central nervous system that process sensory information. The expression levels of ROR∫ oscillate in the retina and pineal gland with a circadian rhythm. In Neuro2A cells, ROR∫ binds DNA and efficiently directs transcription. The gene encoding human ROR∫ maps to chromosome 9q22. ROR© comprises a 560 amino acid protein that shares 50% amino acid identity with RORå and is most highly expressed in skeletal muscle. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| recommended for detection of RORβ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 241-282 mapping within an internal region of ROR&beta of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 241-282 mapping within an internal region of RORβ of human origin |
IHC
The immunostaining of the 3 melatonin nuclear receptors RORα (A, D), RORβ (B, E) and RORγ (C, F) in the spleen of broilers at 14 D.| Application Notes | recommended for detection of RORβ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
