Application
Western blot analysis of Cytokeratin 20 expression in CCRF-CEM whole cell lysate.Application
Western blot analysis of Cytokeratin 20 expression human colon tissue extract.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.| Product Name | Cytokeratin 20 Antibody (H-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-70 mapping at the N-terminus of cytokeratin 20 of human origin |
| Modification Notes | Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epi-thelial cells. The a-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins are useful markers of tissue differentiation, and in addition, they aid in the characterization of malignant tumors. Cytokeratin 20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract, and Cytokeratin 20 is a useful marker of pancreatic and colorectal cancer. Cytokeratin 20 is also helpful in distinguishing different types of highly related carcinomas, such as renal oncocytomas from renal cell carcinomas. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Cytokeratin 20 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-70 mapping at the N-terminus of cytokeratin 20 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-70 mapping at the N-terminus of cytokeratin 20 of human origin |
| Entrez Gene | 54474 |
|---|
Application
Western blot analysis of Cytokeratin 20 expression in CCRF-CEM whole cell lysate.
Application
Western blot analysis of Cytokeratin 20 expression human colon tissue extract.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.| Application Notes | recommended for detection of Cytokeratin 20 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
