Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.| Product Name | p-Flk-1 Antibody (Tyr 1175) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Tyr 1175 of Flk-1 of human origin |
| Modification | Phosphoralated |
| Modification Notes | Three cell membrane receptor tyrosine kinases, Flt (also designated VEGF-R1) (1–3), Flk-1 (also designated VEGF-R2) (4–6) and Flt-4 (7), putatively involved in the growth of endothelial cells, are characterized by the presence of seven immunoglobulin-like sequences in their extracellular domain. These receptors exhibit high degrees of sequence homology to each other as well as lesser degrees of homology to the class III receptors, including CSF-1/Fms, PDGR, SLFR/Kit and Flt-3/Flk-2. Two members of this receptor class, Flt-1 and Flk-1, have been shown to represent high affinity receptors for vascular endothelial growth factors (VEGFs) (3,5). In response to VEGF binding, Flk-1 undergoes autophosphorylation in the kinase insert domain on Tyr 951 and Tyr 996 and in the tyrosine kinase catalytic domain on Tyr 1054 and Tyr 1059 (7,8). Upon activation, Flk-1 recruits several adapter proteins, including Shc, Grb2, Nck and protein tyrosine phosphatases SHP-1 and SHP-2 (9). The mediation of VEGF signaling by Flk-1 promotes proliferation, chemotaxis, prouting and angiogenesis (10). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHC |
| recommended for detection of Tyr 1175 phosphorylated Flk-1 of human origin, correspondingly phosphorylated Tyr 1173 Flk-1 of mouse origin and correspondingly phosphorylated Tyr 1171 of rat origin by IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Tyr 1175 of Flk-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Tyr 1175 of Flk-1 of human origin |
| Entrez Gene | 3791 |
|---|
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.| Application Notes | recommended for detection of Tyr 1175 phosphorylated Flk-1 of human origin, correspondingly phosphorylated Tyr 1173 Flk-1 of mouse origin and correspondingly phosphorylated Tyr 1171 of rat origin by IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
