Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of COL6A1 expression in C3H/10T1/2 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse uterus tissue showing extracellular matrix and nuclear localization.Application
Western blot analysis of COL6A1 expression in CCD-1064Sk (A) and Hs 732 Sk/Mu (B) whole cell lysates.| Product Name | COL6A1 Antibody (H-200) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 51-250 mapping near the N-terminus of Collagen α1 Type VI of human origin |
| Modification Notes | The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Collagen α1 Type VI of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 51-250 mapping near the N-terminus of Collagen &alpha 1 Type VI of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 51-250 mapping near the N-terminus of Collagen α1 Type VI of human origin |
| Entrez Gene | 1291 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of COL6A1 expression in C3H/10T1/2 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse uterus tissue showing extracellular matrix and nuclear localization.
Application
Western blot analysis of COL6A1 expression in CCD-1064Sk (A) and Hs 732 Sk/Mu (B) whole cell lysates.| Application Notes | recommended for detection of Collagen α1 Type VI of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
