Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing cytoplasmic staining of respiratory epithelial cells.Application
Western blot analysis of CHMP1A expression in non-transfected: (A) and mouse CHMP1A transfected: (B) 293T whole cell lysates.| Product Name | CHMP1A Antibody (FL-196) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-196 representing full length CHMP1A of human origin |
| Modification Notes | The charged multivesicular body proteins or chromatin modifying proteins, commonly designated CHMPs, belong to the vacuolar sorting protein family and function as chromatin-modifying proteins. CHMP1-6 are all components of ESCRT (endosomal sorting complex required for transport) I, II or III complexes. These complexes are crucial for sorting endosomal articles into multivesicular bodies (MVBs), as well as required for the formation of these bodies. CHMP1 interacts with VPS4B and localizes to early endosomes. Two isoforms, encoded by distinct genes, exists for CHMP1. They are designated CHMP1A and CHMP1B. CHMP1 overexpression can lead to the dilation of endosomal compartments resulting in the disruption of normal distribution of endosomal markers. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CHMP1A, and to a lesser extent, CHMP1B of human, mouse and, to a lesser extent, rat origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-196 representing full length CHMP1A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-196 representing full length CHMP1A of human origin |
| Entrez Gene | 5119 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing cytoplasmic staining of respiratory epithelial cells.
Application
Western blot analysis of CHMP1A expression in non-transfected: (A) and mouse CHMP1A transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of CHMP1A, and to a lesser extent, CHMP1B of human, mouse and, to a lesser extent, rat origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
