Application
Western blot analysis of CYP27B1 expression in non-transfected: (A) and mouse CYP27B1 transfected: (B) 293T whole cell lysates and mouse kidney tissue extract (C).Application
Western blot analysis of CYP27B1 expression in mouse kidney tissue extract.| Product Name | CYP27B1 Antibody (H-90) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 221-310 mapping within an internal region of CYP27B1 of human origin |
| Modification Notes | The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. P450 enzymes are classified into subfamilies based on their sequence similarities. CYP27B1, a 508-amino acid protein that belongs to the XXVIIB subfamily of the cytochrome P450 family, localizes to the mitochondrion and is expressed in the kidney. The CYP27B1 protein catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D) to 1-alpha,25-dihydroxyvitamin D3 (1,25(OH)2D) and functions in calcium metabolism, normal bone growth, and tissue differentiation. Mutations in the gene which encodes for CYP27B1 cause vitamin D-dependent rickets type 1 (VDDR-1), also designated pseudovitamin D deficiency rickets (PDDR), an autosomal recessive disease characterized by early onset of rickets with hypocalcemia and muscle weakness. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CYP27B1 of human, mouse and, to a lesser extent, rat origin by WB, IP, IF and ELISA also reactive with additional species, including porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 221-310 mapping within an internal region of CYP27B1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 221-310 mapping within an internal region of CYP27B1 of human origin |
| Entrez Gene | 1594 |
|---|
Application
Western blot analysis of CYP27B1 expression in non-transfected: (A) and mouse CYP27B1 transfected: (B) 293T whole cell lysates and mouse kidney tissue extract (C).
Application
Western blot analysis of CYP27B1 expression in mouse kidney tissue extract.| Application Notes | recommended for detection of CYP27B1 of human, mouse and, to a lesser extent, rat origin by WB, IP, IF and ELISA also reactive with additional species, including porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
