Application
Antibodies tested include p-GABAB R2 (Ser 893): (A, B) and GABAB R2 (E-16): (C, D).Application
Western blot analysis of GABAB R2 phosphorylation in untreated (A) and lambda protein phosphatase treated (B) mouse brain tissue extracts.Application
Western blot analysis of GABAB R2 phosphorylation in rat GABAB R2 transfected (A,C) and non-transfected (B,D) COS-7 whole cell lysates. Lanes C and D were treated with lambda protein phosphatase.| Product Name | p-GABAB R2 Antibody (Ser 893) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ser 893 phosphorylated GABAB R2 of human origin and correspondingly Ser 892 phosphorylated GABAB R2 of mouse and rat origin by WB, IP and IF; also reactive with additional species, including bovine and canine |
| Modification | Phosphoralated |
| Modification Notes | GAD-65 and GAD-67, glutamate decarboxylases, catalyze the production of GABA (gamma-aminobutyric acid). In the central nervous system GABA (gamma-aminobutyric acid) acts as the main inhibitory transmitter by increasing a Cl-- conductance that inhibits neuronal firing. GABA activates both ionotropic (GABAA) and metabotropic (GABAB) receptors as well as a third class of receptors called GABAC. Members of the GABAA receptor family include GABAA Rå1-6, GABAA R ∫1-3, GABAA R©1-3, GABAA R∂, GABAA Ré, GABAA Rρ1 and GABAA Rρ2. The GABAB family is composed of GABAB R1å and GABAB R1∫. PKA phosphorylates GABABR2 at Ser 893. This phosphorylation appears to enhance the membrane stability of GABAB R2 . |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIF |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 893 phosphorylated GABAB R2 of human origin and correspondingly Ser 892 phosphorylated GABAB R2 of mouse and rat origin by WB IP and IF also reactive with additional species including bovine and canine |
|---|---|
| Tissue Specificity | recommended for detection of Ser 893 phosphorylated GABAB R2 of human origin and correspondingly Ser 892 phosphorylated GABAB R2 of mouse and rat origin by WB, IP and IF; also reactive with additional species, including bovine and canine |
| Entrez Gene | 9568 |
|---|
Application
Antibodies tested include p-GABAB R2 (Ser 893): (A, B) and GABAB R2 (E-16): (C, D).
Application
Western blot analysis of GABAB R2 phosphorylation in untreated (A) and lambda protein phosphatase treated (B) mouse brain tissue extracts.
Application
Western blot analysis of GABAB R2 phosphorylation in rat GABAB R2 transfected (A,C) and non-transfected (B,D) COS-7 whole cell lysates. Lanes C and D were treated with lambda protein phosphatase.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
