Application
Western blot analysis of NCK1 expression in non-transfected: (A) and human NCK1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of NCK1 expression in non-transfected: (A) and mouse NCK1 transfected: (B) 293T whole cell lysates.| Product Name | NCK1/2 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of NCK1 of human origin |
| Modification Notes | The NCK family of SH2/SH3 adaptor proteins consists of two members, NCK1 (NCKå) and NCK2 (NCK∫), which couple tyrosine kinase signaling, including the EGF and PDGF receptor-pathways, to downstream signaling proteins. Specifically, overexpression of Nck1 in NIH/3T3 cells decreases DNA synthesis stimulated by EGF. Furthermore, the SH2 domain of NCK2 inhibits EGF- and PDGF-induced DNA synthesis. The SH3 domain of NCK binds a proline-rich domain on PAK, a known actin cytoskeleton regulator. The NCK protein thus mediates the interaction between PAK and RAC. The NCK2 protein binds human PDGFR-b (Tyr-1009); overexpression of Nck2 inhibits PDGF-induced membrane ruffling and lamellipod formation. Various growth factor receptors, cell surface antigens and adhesion molecules phosphorylate mammalian NCK1 and NCK2. The human NCK1 and NCK2 genes map to chromosomes 3q21 and 2q12, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NCK1 and NCK2 isoform B, and to a lesser extent, NCK2 isoform A of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of NCK1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of NCK1 of human origin |
| Entrez Gene | 4690 |
|---|
Application
Western blot analysis of NCK1 expression in non-transfected: (A) and human NCK1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of NCK1 expression in non-transfected: (A) and mouse NCK1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of NCK1 and NCK2 isoform B, and to a lesser extent, NCK2 isoform A of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
