Application
Western blot analysis of COUP-TF/EAR2 expression in Hep G2 whole cell lysate.Application
Western blot analysis of COUP-TF/EAR2 expression in Ramos whole cell lysate.| Product Name | COUP-TF/EAR2 Antibody (H-230) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 121-350 mapping within an internal region of EAR2 of human origin |
| Modification Notes | COUP (chicken ovalbumin upstream promoter) transcription factors have been cloned in several species and identified as orphan members of the steroid/thyroid hormone receptor superfamily. COUP-TFI (also designated COUP or EAR-3) and ARP-1 (also designated COUP-TFII) exhibit highly regulated and overlapping expression in most tissues. COUP-TFs are highly expressed in the developing and central nervous system, suggesting that these factors may be important in neural development and differentiation. COUP-TFs can compete for binding to response elements which are common to other members of this family, including RAR, RXR, PPAR, HNF-4, VDR and TR. They have been shown to act as negative regulators as well as initiators of transcription. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of COUP-TFI, ARP-1 and EAR2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 121-350 mapping within an internal region of EAR2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 121-350 mapping within an internal region of EAR2 of human origin |
| Entrez Gene | 2063 |
|---|
Application
Western blot analysis of COUP-TF/EAR2 expression in Hep G2 whole cell lysate.
Application
Western blot analysis of COUP-TF/EAR2 expression in Ramos whole cell lysate.| Application Notes | recommended for detection of COUP-TFI, ARP-1 and EAR2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
