Application
Western blot analysis of CD7 expression in CCRF-CEM whole cell lysate.Application
Western blot analysis of CD7 expression in non-transfected control (A) and CD7 siRNA transfected (B) HeLa cells. Blot probed with CD7 (H-126): . GAPDH (FL-335): used as specificity and loading control.Application
Western blot analysis of CD7 expression in Jurkat whole cell lysate.| Product Name | CD7 Antibody (H-126) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 27-153 of CD7 of human origin |
| Modification Notes | CD7 is a type I transmembrane glycoprotein that is expressed on pluripotential hemapoietic cells, most human thymocytes and some peripheral blood T cells (1,2). CD7 is a marker for pluripotential stem cell leukemias and T cell acute lymphocytic leukemia (3). A role for CD7 in the activation of T cells with g/d receptors has been suggested (4,5). CD8 T cells from patients infected with HIV-1 displayed profound down-modulation of CD7 expression as compared with healthy subjects (6). CD7 is among the pan-T-cell antigens down-regulated in acute infectious mononucleosis. (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CD7 of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 27-153 of CD7 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 27-153 of CD7 of human origin |
| Entrez Gene | 924 |
|---|
Application
Western blot analysis of CD7 expression in CCRF-CEM whole cell lysate.
Application
Western blot analysis of CD7 expression in non-transfected control (A) and CD7 siRNA transfected (B) HeLa cells. Blot probed with CD7 (H-126): . GAPDH (FL-335): used as specificity and loading control.
Application
Western blot analysis of CD7 expression in Jurkat whole cell lysate.| Application Notes | recommended for detection of CD7 of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
