Application
Western blot analysis of GFRα-2 expression in non-transfected 293T: (A) and human GFRα-2 transfected 293T: (B) whole cell lysates and rat testis (C) tissue extract.| Product Name | GFRα-2 Antibody (H-89) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 356-444 mapping near the C-terminus of GFRα-2 of human origin |
| Modification Notes | Glial cell line-derived neurotrophic factor (GDNF) and the related neurotrophic factor neurturin (NTN) are potent survival factors for central and peripheral neurons. GDNF is a glycosylated, disulfide-bonded homodimer that is distantly related to the TGF∫ superfamily of growth factors. Three receptors for these factors, GFRå-1 (also designated GDNFR-å, RETL1 or TrnR-1), GFRå-2 (also designated GDNFR-∫, RETL2, NTNR-å or TrnR-2) and GFRå-3 have been identified. The receptors do not contain transmembrane domains and are attached to the cell membrane by glycosyl-phosphoinositol linkage. Both GFRå-1 and GFRå-2 have been shown to mediate the GDNF-dependent and NTN-dependent phosphorylation and activation of the tyrosine kinase Ret. GFRå-3 is expressed only during development. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of precursor and mature forms of GFRα-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 356-444 mapping near the C-terminus of GFR&alpha -2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 356-444 mapping near the C-terminus of GFRα-2 of human origin |
| Entrez Gene | 2675 |
|---|
Application
Western blot analysis of GFRα-2 expression in non-transfected 293T: (A) and human GFRα-2 transfected 293T: (B) whole cell lysates and rat testis (C) tissue extract.| Application Notes | recommended for detection of precursor and mature forms of GFRα-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
