Application
Western blot analysis of RBP-Jκ expression in non-transfected: (A) and human RBP-Jκ transfected: (B) 293T whole cell lysates.| Product Name | RBP-Jκ Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 451-500 mapping at the C-terminus of RBP-Jκ of human origin |
| Modification Notes | Recombination signal binding protein J˚ (RBP-J˚), also designated KBF2 or CBF1, is the mammalian homolog of the Drosophila Suppressor of Hairless [Su(H)], a protein involved in the development of the peripheral nervous system. RBP-J˚ is ubiquitously expressed in mammalian tissues and is involved in the regulation of gene expression. RBP-J˚ has been shown to directly interact with the intercellular domain of the cell surface receptor Notch 1. Proteolytically cleaved Notch 1 translocates to the nucleus, where it binds DNA-bound RBP-J˚ and activates transcription of target genes. These genes include NF˚B p52 and the Epstein-Barr virus (EBV) protein EBNA-2, both of which contain RBP-J˚-binding sequences within their promoter regions. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of RBP-Jκ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 451-500 mapping at the C-terminus of RBP-J&kappa of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 451-500 mapping at the C-terminus of RBP-Jκ of human origin |
| Entrez Gene | 3516 |
|---|
Application
Western blot analysis of RBP-Jκ expression in non-transfected: (A) and human RBP-Jκ transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of RBP-Jκ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
