Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of human recombinant MMP-1.| Product Name | MMP-1/8 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 100-399 mapping within an internal region of MMP-1 of human origin |
| Modification Notes | The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-9 (also designated 92 kDa type IV collagenase or gelatinase B) has been shown to degrade bone collagens in concert with MMP-1 (also designated interstitial collagenase, fibroblast collagenase or collagenase-1) and cysteine proteases, and may play a role in bone osteoclastic resorption. MMP-1 is downregulated by p53, and abnormality of p53 expression may contribute to joint degradation in rheumatoid arthritis by regulating MMP-1 expression. MMP-8 (also designated neutrophil collagenase, PMNL collagenase or collagenase-2) degrades fibrillar collagen types I, II and III. Unlike other members of the MMP family, MMP-8 is expressed exclusively in inflammatory conditions. MMP-8 is highly expressed in the postpartum uterus, and it is thought to be involved in the postpartum involution of the uterus. MMP-8 is also the predominant collagenase expressed in ulcers and healing wounds. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of MMP-1 of human origin and MMP-8 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA partially cross-reactive with other MMP family members also reactive with additional species, including equine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 100-399 mapping within an internal region of MMP-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 100-399 mapping within an internal region of MMP-1 of human origin |
| Entrez Gene | 4312 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of human recombinant MMP-1.| Application Notes | recommended for detection of MMP-1 of human origin and MMP-8 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA partially cross-reactive with other MMP family members also reactive with additional species, including equine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
