Application
Western blot analysis of full length mouse recombinant dsg1.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skin tissue showing membrane staining of epidermal and adnexal cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of normal mouse heart frozen section showing cell junction staining.Application
Western blot analysis of dsg1 expression in F9 whole cell lysate.Application
Western blot analysis of dsg1 expression in A-375 whole cell lysate.| Product Name | dsg1 Antibody (H-290) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 760-1049 mapping near the C-terminus of dsg1 of human origin |
| Modification Notes | Desmogleins (dsgs) are type I membrane proteins that are important for cell adhesion and are expressed in great abundance at the desmosomes, which are adhesive cell junctions. The dsg proteins belong to the cadherin family and consist of dsg1, dsg2 and dsg3. Calcium binds to the putative calcium binding sites at the extracellular N-terminal domain of dsg1, which has cadherin-like repeats. Unlike normal human keratinocytes, the squamous cell carcinoma cells exhibit diminished or unusual expression of dsg3 and dsg1, which bear pemphigus vulgaris and pemphigus foliaceus antigens, respectively. Cultured normal human keratinocytes express dsg1 and dsg3 mRNA, with or without dsg 2 mRNA, which indicates that desmoglein isoforms exhibit abnormal expression and may be related to tumor cell kinetics, such as cell invasion and metastasis. Pemphigus is an autoimmune disease of skin adhesion associated with auto-antibodies against a number of keratinocyte antigens, such as the adhesion molecules dsg 1 and 3 and acetylcholine receptors. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of dsg1 of human and rat origin and dsg1α, β and γ of mouse origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 760-1049 mapping near the C-terminus of dsg1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 760-1049 mapping near the C-terminus of dsg1 of human origin |
| Entrez Gene | 1828 |
|---|
Application
Western blot analysis of full length mouse recombinant dsg1.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skin tissue showing membrane staining of epidermal and adnexal cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of normal mouse heart frozen section showing cell junction staining.
Application
Western blot analysis of dsg1 expression in F9 whole cell lysate.
Application
Western blot analysis of dsg1 expression in A-375 whole cell lysate.| Application Notes | recommended for detection of dsg1 of human and rat origin and dsg1α, β and γ of mouse origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
