Application
Western blot analysis of GPVI expression in MEG-01 (A), SK-N-SH (B), SJRH30 (C), IMR-32 (D) and SH-SY5Y (E) whole cell lysates.Application
Western blot analysis of GPVI expression in MEG-01 (A), Jurkat (B) and K-562 (C) whole cell lysates.| Product Name | GPVI Antibody (H-139) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | corresponding to amino acids 201-339 mapping at the C-terminus of GPVI-1 of human origin |
| Modification Notes | CD32 (also designated Fc gamma RII) is a low affinity receptor for the Fc fragment of aggregated IgG. CD32 is responsible for the clearance of immunocomplexes by macrophages and also plays an important role in the regulation of antibody production by B cells. A member of the immunoglobulin superfamily, glycoprotein VI (GPVI), is a collagen receptor that plays a critical role in collagen-induced platelet aggregation. Patients who are deficient in GPVI suffer from bleeding disorders, and GPVI may be involved with cardiovascular and cerebral vascular diseases. GPVI also binds the collagen related peptide (CRP) and convulxin (Cvx), a GPVI-specific ligand from snake venom. GPVI mediates its signal through CD32, which in response to Cvx, leads to tyrosine phosphorylation and activation of Syk and PLCg2. The gene encoding human GPVI maps to chromosome 19q13 and produces three isoforms, full length GPVI-1 and two additional isoforms, GPVI-2 and GPVI-3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GPVI-1, -2 and -3 of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | corresponding to amino acids 201-339 mapping at the C-terminus of GPVI-1 of human origin |
|---|---|
| Tissue Specificity | corresponding to amino acids 201-339 mapping at the C-terminus of GPVI-1 of human origin |
| Entrez Gene | 51206 |
|---|
Application
Western blot analysis of GPVI expression in MEG-01 (A), SK-N-SH (B), SJRH30 (C), IMR-32 (D) and SH-SY5Y (E) whole cell lysates.
Application
Western blot analysis of GPVI expression in MEG-01 (A), Jurkat (B) and K-562 (C) whole cell lysates.| Application Notes | recommended for detection of GPVI-1, -2 and -3 of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
