Application
Western blot analysis of V-ATPase B1 expression in non-transfected: (A) and mouse V-ATPase B1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of V-ATPase B1/2 expression in mouse kidney tissue extract.Application
Western blot analysis of V-ATPase B1 expression in non-transfected 293T: (A), human V-ATPase B1 transfected 293T: (B) and JAR (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomeruli and tubuli, with strong staining of distal tubules at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of V-ATPase B1/2 expression in JAR whole cell lysate.| Product Name | V-ATPase B1/2 Antibody (H-180) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 334-513 mapping at the C-terminus of V-ATPase B1 of human origin |
| Modification Notes | Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis, and a integral V0 domain, which is responsible for proton translocation, compose V-ATPase. Nine subunits (A–H) make up the V1 domain and five subunits (a, d, c, c' and c") make up the V0 domain. Like F-ATPase, V-ATPase most likely operates through a rotary mechanism. The V-ATPase V1 B subunit exists as two isoforms. In the inner ear, the V-ATPase B1 isoform functions in proton secretion and is required to maintain proper endolymph pH and normal auditory function. The gene encoding the human V-ATPase B1 isoform maps to chromosome 2cen-q13. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness. The V-ATPase B2 isoform is expressed in kidney and is the only B isoform expressed in osteoclasts. The gene encoding the human V-ATPase B2 isoform maps to chromosome 8p22-p21. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of V-ATPase B1 and 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 334-513 mapping at the C-terminus of V-ATPase B1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 334-513 mapping at the C-terminus of V-ATPase B1 of human origin |
| Entrez Gene | 525 |
|---|
Application
Western blot analysis of V-ATPase B1 expression in non-transfected: (A) and mouse V-ATPase B1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of V-ATPase B1/2 expression in mouse kidney tissue extract.
Application
Western blot analysis of V-ATPase B1 expression in non-transfected 293T: (A), human V-ATPase B1 transfected 293T: (B) and JAR (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomeruli and tubuli, with strong staining of distal tubules at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of V-ATPase B1/2 expression in JAR whole cell lysate.| Application Notes | recommended for detection of V-ATPase B1 and 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
