Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of glandular cells and islet of langerhans cells.Application
Western blot analysis of IL-3/IL-5/GM-CSFRβ expression in non-transfected: (A) and human IL-3/IL-5/GM-CSFRβ transfected: (B) 293T whole cell lysates.Application
Western blot analysis of IL-3/IL-5/GM-CSFRβ expression in THP-1 (A) and RAW 264.7 (B) whole cell lysates.| Product Name | IL-3/IL-5/GM-CSFRβ Antibody (K-17) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of IL-3/IL-5/GM-CSFRβ of mouse origin |
| Modification Notes | The human IL-3, IL-5 and GM-CSF receptors are each composed of both unique a subunits and a common ∫ subunit. The a subunits are low affinity ligand binding proteins while the ∫ subunits do not themselves bind ligand, but are required for high affinity binding by the a subunits (1–5). In contrast, the mouse IL-3 receptor has two distinct ∫ subunits, one that functions only in IL-3 mediated cell signaling and a second that is shared with IL-5 and GM-CSF (6). The murine ∫-subunits are 91% homologous at the amino acid level but only 56% homologous to the human ∫ subunit (6). Although neither the murine nor the human ∫ subunit contains tyrosine kinase domains, both activate tyrosine phosphorylation mediated signaling pathways (7,8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of β chain common to IL-3R, IL-5R and GM-CSFR of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of IL-3/IL-5/GM-CSFR&beta of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of IL-3/IL-5/GM-CSFRβ of mouse origin |
| Entrez Gene | 1439 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of glandular cells and islet of langerhans cells.
Application
Western blot analysis of IL-3/IL-5/GM-CSFRβ expression in non-transfected: (A) and human IL-3/IL-5/GM-CSFRβ transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of IL-3/IL-5/GM-CSFRβ expression in THP-1 (A) and RAW 264.7 (B) whole cell lysates.| Application Notes | recommended for detection of β chain common to IL-3R, IL-5R and GM-CSFR of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
