Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal cells.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human epididymis tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Trk B expression in rat brain tissue extract.Application
Western blot analysis of human recombinant Trk B.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing membrane localization.| Product Name | Trk B Antibody (H-181) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 160-340 mapping within the extracellular domain of Trk B of human origin |
| Modification Notes | The Trk proto-oncogene encodes a tyrosine protein kinase, Trk A, also designated Trk gp140, that serves as a receptor for certain neurotrophic factors including nerve growth factor (NGF) and neurotrophin-3 (NT-3). Trk B is a tyrosine kinase gene highly related to Trk A. Trk B expression is confined to tissues within the central and peripheral nervous systems. The brain-derived neurotrophic factor (BDNF) and NT-3, but not NGF, can induce rapid phosphorylation on tyrosine of Trk B gp145, one of the receptors encoded by NTRK2, although BDNF elicits a response at least two orders of magnitude greater than NT-3. Thus it appears that Trk B gp145 may represent a neurotrophic receptor for BDNF and NT-3. The third member of the Trk family of tyrosine kinases, Trk C, encodes a protein designated Trk C gp145 that is preferentially expressed in brain tissue, is equally related to Trk A and Trk B and is a functional receptor for NT-3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Trk B splice variants L1 and L10 of mouse origin, Trk B gp95 and Trk B gp145 of rat origin and Trk B, Trk B-T1 and Trk B T-Shc of human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 160-340 mapping within the extracellular domain of Trk B of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 160-340 mapping within the extracellular domain of Trk B of human origin |
| Entrez Gene | 4915 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal cells.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human epididymis tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Trk B expression in rat brain tissue extract.
Application
Western blot analysis of human recombinant Trk B.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing membrane localization.| Application Notes | recommended for detection of Trk B splice variants L1 and L10 of mouse origin, Trk B gp95 and Trk B gp145 of rat origin and Trk B, Trk B-T1 and Trk B T-Shc of human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
