Application
Western blot analysis of PP2A-Aα/β expression in Jurkat whole cell lysate.Application
Western blot analysis of PP2A-Aα/β expression in KNRK whole cell lysate.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.| Product Name | PP2A-Aα/β Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 290-589 mapping at the C-terminus of PP2A-Aα of human origin |
| Modification Notes | In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family. The PP2A family comprises subfamily members PP2Aå and PP2A∫. The PP2A catalytic subunit associates with a variety of regulatory subunits. Regulatory subunits include PP2A-Aå and -A∫, PP2A-Bå and -B∫, PP2A-Cå and -C∫ and PP2A-B56å and -B56∫. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PP2A-Aα and PP2A-Aβ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 290-589 mapping at the C-terminus of PP2A-A&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 290-589 mapping at the C-terminus of PP2A-Aα of human origin |
| Entrez Gene | 5518 |
|---|
Application
Western blot analysis of PP2A-Aα/β expression in Jurkat whole cell lysate.
Application
Western blot analysis of PP2A-Aα/β expression in KNRK whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.| Application Notes | recommended for detection of PP2A-Aα and PP2A-Aβ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
