Application
Western blot analysis of uricase expression in non-transfected: (A) and mouse uricase transfected: (B) 293T whole cell lysates and mouse liver tissue extract (C).Application
Western blot analysis of uricase expression in mouse liver tissue extract.| Product Name | uricase Antibody (FL-303) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-303 representing full length uricase of mouse origin |
| Modification Notes | In most mammals, urate oxidase (uricase) is present in liver, with little or no detectable activity in other tissues. It is associated with the peroxisomes and exists as a tetramer. Humans and certain primates lack this enzyme, which catalyzes the oxidation of uric acid to allantoin. The human Lesch-Nyhan syndrome is a rare neurological and behavioral disorder caused by an inherited deficiency in the level of activity of the purine salvage enzyme hypoxanthine-guanosine phosphoribosyl transferase (HPRT). The identification of mice with complete HPRT deficiency but without any symptoms of the Lesch-Nyhan syndrome raises the possibility that the absence of uricase activity in the purine metabolism pathway may contribute to the development of the neurologic symptoms observed in humans. Comparison of the sequences in man, mouse and pig suggested that loss of uricase function in man was due to a sudden mutational event. The gene which encodes uricase maps to human chromosome 1p22. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of uricase of mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-303 representing full length uricase of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-303 representing full length uricase of mouse origin |
| Entrez Gene | 22262 |
|---|
Application
Western blot analysis of uricase expression in non-transfected: (A) and mouse uricase transfected: (B) 293T whole cell lysates and mouse liver tissue extract (C).
Application
Western blot analysis of uricase expression in mouse liver tissue extract.| Application Notes | recommended for detection of uricase of mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
