Application
Western blot analysis of OATP-B expression in JAR (A), HeLa (B) and Hep G2 (C) whole cell lysates.| Product Name | OATP-B Antibody (H-189) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 521-709 mapping at the C-terminus of OATP-B of human origin |
| Modification Notes | The organic anion transporter family of proteins mediate hepatic uptake of cardiac glycosides. OATP-A and OATP-C are both pravastatin transporters, suggesting that they are responsible for the hepatic uptake of the liver-specific hydroxymethylglutaryl-CoA reductase inhibitor in mouse, rat and human. OATP-A is expressed in liver and kidney and helps mediate sodium-independent uptake of the anionic steroid conjugates dehydroepiandrosterone sulfate, estradiol-17-glucuronide and prostaglandin. OATP-C is exclusively expressed in liver and is localized to the basolateral hepatocyte membrane. OATP-B, also known as Slco2b1 or Slc21a9, mediates the NA+ independent transport of organic anions such as taurocholate, leukotriene C4, thromboxane B2 and iloprost during the absorption of bile acids in the liver. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of OATP-B of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 521-709 mapping at the C-terminus of OATP-B of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 521-709 mapping at the C-terminus of OATP-B of human origin |
Application
Western blot analysis of OATP-B expression in JAR (A), HeLa (B) and Hep G2 (C) whole cell lysates.| Application Notes | recommended for detection of OATP-B of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
