Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear and cytoplasmic staining of squamous epithelial cells.Application
Western blot analysis of cyclin D1 expression in non-transfected: (A) and human cyclin D1 transfected: (B) 293T whole cell lysates.Application
Antibodies tested include cyclin D1 (H-295): (A,B) and cyclin D1 (M-20): (C,D).Application
Immunofluorescence staining of methanol-fixed C32 cells showing nuclear localization.| Product Name | cyclin D1 Antibody (M-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of cyclin D1 of human origin |
| Modification Notes | The proliferation of eukaryotic cells is controlled at specific points in the cell cycle, particularly at the G1 to S and the G2 to M transitions. It is well established that the Cdc2 p34-cyclin B protein kinase plays a critical role in the G2 to M transition while cyclin A associates with Cdk2 p33 and functions in S phase. Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin E. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of cyclin D1 and, to a lesser extent, cyclin D2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA non cross-reactive with cyclin D3: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of cyclin D1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of cyclin D1 of human origin |
| Entrez Gene | 595 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear and cytoplasmic staining of squamous epithelial cells.
Application
Western blot analysis of cyclin D1 expression in non-transfected: (A) and human cyclin D1 transfected: (B) 293T whole cell lysates.
Application
Antibodies tested include cyclin D1 (H-295): (A,B) and cyclin D1 (M-20): (C,D).
Application
Immunofluorescence staining of methanol-fixed C32 cells showing nuclear localization.| Application Notes | recommended for detection of cyclin D1 and, to a lesser extent, cyclin D2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA non cross-reactive with cyclin D3: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
