Application
Western blot analysis of Ran GAP1 expression in non-transfected 293T: (A), mouse Ran GAP1 transfected 293T: (B) and NTERA-2 cl.D1 (C) whole cell lysates.Application
Western blot analysis of Ran GAP1 expression in non-transfected: (A) and human Ran GAP1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Ran GAP1 expression in non-transfected: (A) and mouse Ran GAP1 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear envelope localization.Application
Western blot analysis of Ran GAP1 expression of the cytoplasmic and nuclear envelope associated forms in MCF7 whole cell lysate.| Product Name | Ran GAP1 Antibody (H-180) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 408-587 mapping at the C-terminus of Ran GAP1 of human origin |
| Modification Notes | The small Ras related protein Ran, also called TC4, is a nuclear localized GTPase implicated in a diverse array of cellular processes including DNA replication, entry into and exit from mitosis and the transport of RNA and proteins through the nuclear pore complex. Like Ras, active Ran GTP and inactive Ran GDP levels are tightly regulated by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The abundant GEF RCC1 (regulator of chromosome condensation 1) increases the rate at which Ran exchanges GDP for GTP. Ran GAP1 opposes the effects of RCC1 by increasing the rate at which Ran hydrolyzes GTP to GDP. A protein designated Ran BP1 has no intrinsic GAP activity and functions as a GEF inhibitor deactivating RCC1 and thereby indirectly increasing the ratio of Ran GDP to Ran GTP. Ran BP2 has been proposed as the Ran GTP docking site at the periphery of the nuclear pore complex. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ran GAP1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 408-587 mapping at the C-terminus of Ran GAP1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 408-587 mapping at the C-terminus of Ran GAP1 of human origin |
| Entrez Gene | 5905 |
|---|
Application
Western blot analysis of Ran GAP1 expression in non-transfected 293T: (A), mouse Ran GAP1 transfected 293T: (B) and NTERA-2 cl.D1 (C) whole cell lysates.
Application
Western blot analysis of Ran GAP1 expression in non-transfected: (A) and human Ran GAP1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Ran GAP1 expression in non-transfected: (A) and mouse Ran GAP1 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear envelope localization.
Application
Western blot analysis of Ran GAP1 expression of the cytoplasmic and nuclear envelope associated forms in MCF7 whole cell lysate.| Application Notes | recommended for detection of Ran GAP1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
