Application
Western blot analysis of BF-1 expression in Hep G2 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic localization.Application
Western blot analysis of BF-1 expression in non-transfected 293T: (A), mouse BF-1 transfected 293T: (B) and SK-N-SH (C) whole cell lysates.| Product Name | BF-1 Antibody (O-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of BF-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | The winged-helix transcriptional repressor (WH) BF-1 gene encodes brain factor 1 (BF-1), also known as foxg1, and is essential for the proliferation of progenitor cells in the cerebral cortex and influences regional patterning in the mammalian telencephalon (1–4). WH proteins are a family of putative transcriptional regulators with diverse roles in development, and are characterized by a highly conserved DNA binding structure, the WH domain (1,5,6). BF-1 plays a critical role in the development of the cerebral hemispheres of the brain and targeted disruption of the gene leads to severe defects in the development of telencephalic structures, such as the cerebral cortex and basal ganglia (1). The loss of BF-1 results in an accelerated rate of neuronal differentiation and the shortening of the neurogenetic period in the embryonic cerebral cortex (1,7). BF-1 is expressed by E8.5 in telencephalic progenitors (1). It may also regulate the response of cerebral cortical progenitors to environmental cues (1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of BF-1 of mouse rat and human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of BF-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 2290 |
|---|
Application
Western blot analysis of BF-1 expression in Hep G2 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic localization.
Application
Western blot analysis of BF-1 expression in non-transfected 293T: (A), mouse BF-1 transfected 293T: (B) and SK-N-SH (C) whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
