Application
Western blot analysis of ITF-2 expression in human placenta tissue extract.| Product Name | ITF-2 Antibody (G-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of ITF-2 of mouse and human origin by WB, IP and ELISA |
| Modification Notes | Immunoglobulin transcription factor 2 (ITF-2), also designated transcription factor and SL3-3 enhancer factor 2 (SEF-2) is a basic helix-turn-helix transcription factor. ITF-2 binds to the immunoglobulin enhancer Mu-E5/KE5-motif and to the Ephrussi-box (E-box) element present in SSTR2-INR and serves as an activator of transcription in muscle-specific genes. ITF-2 preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'. ITF-2 belongs to the class of simple bHLH transcription factors identified as ubiquitous E-box binding factors, which also includes the E2A gene products (E12 and E47) and HEB. The protein is expressed in adult heart, brain, placenta, skeletal muscle and embryonic brain. ITF-2 forms homo- or hetero-oligomers with myogenin and MyoD; alternatively spliced isoforms of ITF-2 function to activate or repress their transcription. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of ITF-2 of mouse and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of ITF-2 of mouse and human origin by WB, IP and ELISA |
| Entrez Gene | 6925 |
|---|
Application
Western blot analysis of ITF-2 expression in human placenta tissue extract.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
