Application
Western blot analysis of Photinus pyralis recombinant Luciferase.| Product Name | Luciferase Antibody (251-550) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 251-550 mapping at the C-terminus of Luciferase of Photinus pyralis origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | Luciferase isolated from the common North American firefly, Photinus pyralis, is one of the most extensively studied enzymes that catalyze light production in bioluminescent organisms. Luciferase belongs to the ATP-dependent AMP-binding enzyme family. It produces green light with a wavelength of 562 nM. Following is the chemical catalytic reaction, which is catalyzed by Luciferase: Photinus luciferin + O(2) + ATP = oxidized Photinus luciferin + CO(2) + AMP + diphosphate + light |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIPWB |
| WB:1:100~1:400 IP:1:20~1:100 IF:1:20~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 251-550 mapping at the C-terminus of Luciferase of Photinus pyralis origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 251-550 mapping at the C-terminus of Luciferase of Photinus pyralis origin |
Application
Western blot analysis of Photinus pyralis recombinant Luciferase.| Application Notes | WB:1:100~1:400 IP:1:20~1:100 IF:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
