Application
Western blot analysis of Optineurin expression in Jurkat whole cell lysate.| Product Name | Optineurin Antibody (H-220) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 321-540 mapping near the C-terminus of Optineurin of human origin |
| Modification Notes | Optineurin, also designated FIP2, E3-14.7K-interacting protein, HYPL, transcription factor IIIA-interacting protein (TFIIIA-INTP), Huntingtin interacting protein L and NEMO-related protein, influences cell morphogenesis, membrane trafficking, vesicle trafficking and transcription activation through its interactions with the Rab8, Huntingtin and transcription factor IIIA proteins. Optineurin interacts with adenovirus E3-14.7K protein and may utilize TNFa or FAS-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin mutations may impart normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin is a 617 amino acid protein that contains leucine zippers and leucine-rich regions, and contains a potential Cys2-His-Cys zinc finger at residues 553-582. It localizes to the Golgi apparatus. RT-PCR studies indicate expression in human trabecular meshwork, nonpigmented ciliary epithelium, retina, brain, adrenal cortex, liver, fetus, lymphocyte and fibroblast. Northern blot studies indicate a 2.0 kb transcript in human trabecular meshwork and nonpigmented ciliary epithelium and a minor 3.6 kb transcript. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Optineurin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 321-540 mapping near the C-terminus of Optineurin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 321-540 mapping near the C-terminus of Optineurin of human origin |
Application
Western blot analysis of Optineurin expression in Jurkat whole cell lysate.| Application Notes | recommended for detection of Optineurin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
