Application
Western blot analysis of coilin expression in non-transfected: (A) and human coilin transfected: (B) 293T whole cell lysates.Application
Western blot analysis of coilin expression in Jurkat nuclear extract.Application
Western blot analysis of coilin expression in non-transfected: (A) and mouse coilin transfected: (B) 293T whole cell lysates and MOLT-4 nuclear extract (C).| Product Name | coilin Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 277-576 mapping at the C-terminus of coilin of human origin |
| Modification Notes | Coilin is the primary protein of nuclear coiled (Cajal) bodies. Cajal bodies are small nuclear organelles and contain many proteins involved in RNA transcription and processing. Coilin is a self-associating protein with a nucleolar localization signal. It is essential for the proper formation of Cajal bodies and for the recruitment of snRNP and survival motor neuron (SMN) complex proteins to Cajal bodies. Coilin directly binds SMN proteins in the recruitment process and competes with SmB’ for SMN interactions. In the developing organism, Cajal bodies play a role in the assembly of the nucleolus. While the N-terminus of coilin contains the self-associating domain, the C-terminus of coilin regulates the number of Cajal bodies present in the cell. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of coilin of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 277-576 mapping at the C-terminus of coilin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 277-576 mapping at the C-terminus of coilin of human origin |
| Entrez Gene | 8161 |
|---|
Application
Western blot analysis of coilin expression in non-transfected: (A) and human coilin transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of coilin expression in Jurkat nuclear extract.
Application
Western blot analysis of coilin expression in non-transfected: (A) and mouse coilin transfected: (B) 293T whole cell lysates and MOLT-4 nuclear extract (C).| Application Notes | recommended for detection of coilin of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
