Application
Western blot analysis of DDX11 expression in non-transfected: (A) and human DDX11 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of DDX11 expression in non-transfected: (A) and human DDX11 transfected: (B) 293T whole cell lysates.| Product Name | DDX11 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 405-704 mapping within an internal region of DDX11 of human origin |
| Modification Notes | DEAD-box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure and ribosome/spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX11 (DEAD/H box protein 11), also known as CHLR1 or KRG2, is a member of the DEAD-box protein family and possesses both ATPase and DNA helicase activity. A homolog of the S. cerevisiae CHL1 protein, DDX11 is localized to the nucleus and is highly expressed in the testis, thymus, ovary, spleen and pancreas. DDX11 can bind to both single- and double-stranded DNA and is essential for proper chromosome segregation and embryonic development. Five isoforms of DDX11 exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DDX11 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 405-704 mapping within an internal region of DDX11 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 405-704 mapping within an internal region of DDX11 of human origin |
| Entrez Gene | 1663 |
|---|
Application
Western blot analysis of DDX11 expression in non-transfected: (A) and human DDX11 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of DDX11 expression in non-transfected: (A) and human DDX11 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of DDX11 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
