Application
Western blot analysis of Lamellipodin expression in MG-63 whole cell lysate.| Product Name | Lamellipodin Antibody (H-150) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 91-240 mapping near the N-terminus of Lamellipodin of human origin |
| Modification Notes | Lamellipodin, also called ras-associated and pleckstrin homology domains-containing protein 1 (RAPH1), is a 1302 amino acid member of the MRL family. The peripheral membrane protein mediates localized membrane signals and colocalizes at the tips of filopodia and lamellipodia with ENAH/VASP. Aside from the main isoform, nine additional isoforms have been identified for Lamellipodin (RMO1, RMO1a, RMO1b, RMO1c, RMO1ab, RMO1ac, RMO1bc, RMO1abc and RMO1-RAPH1). RMO1-RAPH1 is expressed in a wide variety of tissues, most highly in brain, heart, ovary and developing embryo. RMO1 is also widely expressed, with highest amounts in liver. Lamellipodin is down regulated in breast and ovarian cancers and shows reduced expression in metastatic osteosarcomas in comparison to primary osteosarcoma tumors. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Lamellipodin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 91-240 mapping near the N-terminus of Lamellipodin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 91-240 mapping near the N-terminus of Lamellipodin of human origin |
| Entrez Gene | 65059 |
|---|
Application
Western blot analysis of Lamellipodin expression in MG-63 whole cell lysate.| Application Notes | recommended for detection of Lamellipodin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
