Application
Western blot analysis of MASP-1/3 expression in rat testis (A), mouse uterus (B), rat liver (C) and mouse liver (D) tissue extracts.| Product Name | MASP-1/3 Antibody (H-260) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 171-430 mapping within an internal region of MASP-1 of human origin |
| Modification Notes | Mannose (or mannan)-binding lectin (MBL), also known as serum mannose-binding protein (MBP), initiates the lectin branch of the innate immune response by binding to the surface of potentially pathogenic microorganisms and initiating complement fixation through an N-terminal collagen-like domain. MBL is a key component in immune response in that it can directly trigger neutralization of invading microorganisms by an Ab-independent mechanism. Mutations of human MBL are associated with immunodeficiency resulting from a reduction in the ability of the mutant MBL to initiate complement fixation. In human, three types of MBL-associated serine proteases, MASP-1, MASP-2 and MASP-3, and a truncated form of MASP-2 (small MBL-associated protein; sMAP or MAp19) complex with MBL to activate the lectin pathway of the complement system. MASP-3 is an alternatively spliced product from the MASP-1 gene. The heavy/A chains are identical between MASP-1 and MASP-3 but the light/B chains are entirely different. Activated MASPs subsequently cleave and activate downstream components of the complement pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MASP-1 and MASP-3 heavy chain of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 171-430 mapping within an internal region of MASP-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 171-430 mapping within an internal region of MASP-1 of human origin |
| Entrez Gene | 5648 |
|---|
Application
Western blot analysis of MASP-1/3 expression in rat testis (A), mouse uterus (B), rat liver (C) and mouse liver (D) tissue extracts.| Application Notes | recommended for detection of MASP-1 and MASP-3 heavy chain of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
