Application
Western blot analysis of PP2Cα expression in non-transfected: (A and human PP2Cα transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PP2Ca/b expression in non-transfected: (A) and human PP2Ca/b transfected: (B) 293T whole cell lysates.| Product Name | PP2Cα/β Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of PP2Cα of human origin |
| Modification Notes | Eukaryotic protein phosphorylation and dephosphorylation on serine and threonine residues regulates numerous cell functions, including division, homeostasis and apoptosis. A group of proteins that play a major role in this process are the serine/threonine protein phosphatases. Protein phosphatase (PP) holoenzyme is a trimeric complex that contains a regulatory subunit, a variable subunit and a catalytic subunit. PP2C family members are negative regulators of cell stress response pathways. Protein phosphatase 2Ca (PP2Ca) has broad specificity. It dephosphorylates and negatively regulates the activities of MAP kinases and MAP kinase-kinases while also inhibiting the activation of p38 and JNK kinase cascades, induced by environmental stresses. PP2Ca also induces the expression of endogenous p53 and the p53-responsive gene p21, leading to cell cycle arrest and apoptosis. The PP2Ca protein, which contains an active site containing a dinuclear metal ion center, shows highest expression in epithelial cells, as well as in the digestive tract, lung, kidney, breast, prostate, endocrine glands and brain. The PP2Cb enzyme also has broad specificity and is highly expressed in the heart and skeletal muscle. It may be involved in cell cycle control as it dephosphorylates the cyclin-dependent kinases (CDKs), CDK2 and CDK6, in vitro. Overexpression of PP2Cb can cause cell-growth arrest or cell death. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PP2Cα isoforms 1 and 2, and PP2Cβof mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of PP2C&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of PP2Cα of human origin |
| Entrez Gene | 5494 |
|---|
Application
Western blot analysis of PP2Cα expression in non-transfected: (A and human PP2Cα transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PP2Ca/b expression in non-transfected: (A) and human PP2Ca/b transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of PP2Cα isoforms 1 and 2, and PP2Cβof mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
