| Product Name | Pmel17 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 25-324 mapping within an N-terminal extracellular domain of Pmel17 of human origin |
| Modification Notes | Cytotoxic T lymphocytes (CTL’s) recognize melanoma-associated antigens, which belong to three main groups. These groups include tumor-associated testis-specific antigens, melanocyte differentiation antigens and mutated or aberrantly expressed antigens, which are routinely used as markers to identify melanomas based on their binding to specific monoclonal antibodies. gp100, also designated ME20-M, ME20-S and PMEL 17, is classified as a melanocyte differentiation antigen and is expressed at low levels in normal cell lines and tissues, but is upregulated in melanocytes. gp100 is a highly glycosylated protein. It is also the product of proteolytic cleavage, which results in a secreted protein. gp100 is recognized by several monoclonal antibodies, including NKI-beteb, HMB-50 and HMB-45, which are used to diagnose melanomas. Therefore, gp100 is considered a potential target for immunotherapy of malignant melanoma. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Pmel17 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 25-324 mapping within an N-terminal extracellular domain of Pmel17 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 25-324 mapping within an N-terminal extracellular domain of Pmel17 of human origin |
| Entrez Gene | 6490 |
|---|
| Application Notes | recommended for detection of Pmel17 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
