Application
Western blot analysis of Pontin 52 expression in SK-BR-3 (A), KNRK (B), NIH/3T3 (C) and A-431 (D) nuclear extracts and NCI-H1299 whole cell lysate (E).Application
Western blot analysis of Pontin 52 expression in SK-BR-3 whole cell lysate.Application
Western blot analysis of Pontin 52 expression in non-transfected: (A) and human Pontin 52 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Pontin 52 expression in K-562 nuclear extract.| Product Name | Pontin 52 Antibody (H-100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 191-290 mapping within an internal region of Pontin 52 of human origin |
| Modification Notes | Pontin 52 is nuclear matrix protein that is primarily expressed in the nucleus and is also present in the cytoplasm. Pontin 52 is expressed in the nucleoplasm of whole cells, but is not present in the nucleoli. Pontin 52 is also designated RUVBL1, for E. coli RuvB-like 1 protein, or NMP 238, and is the human homolog of rat TIP49. Pontin 52 contains an ATPase/helicase motif and may represent a class of cofactors recruited by transcriptional activation domains that function in diverse pathways. For instance, in vivo, Pontin 52 is complexed with Myc and Reptin 52, which is a Pontin 52 related protein also designated RUVBL2. The interaction of Pontin 52 with Myc is dependent upon a Myc domain essential for oncogenic activity, suggesting that functional Pontin 52 is an essential mediator of Myc oncogenic transformation. The gene encoding human Pontin 52 maps to chromosome 3q21. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of Pontin 52 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 191-290 mapping within an internal region of Pontin 52 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 191-290 mapping within an internal region of Pontin 52 of human origin |
| Entrez Gene | 8607 |
|---|
Application
Western blot analysis of Pontin 52 expression in SK-BR-3 (A), KNRK (B), NIH/3T3 (C) and A-431 (D) nuclear extracts and NCI-H1299 whole cell lysate (E).
Application
Western blot analysis of Pontin 52 expression in SK-BR-3 whole cell lysate.
Application
Western blot analysis of Pontin 52 expression in non-transfected: (A) and human Pontin 52 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Pontin 52 expression in K-562 nuclear extract.| Application Notes | recommended for detection of Pontin 52 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
