Application
Western blot analysis of VPS25 expression in K-562 (A) and NIH/3T3 (B) whole cell lysates.| Product Name | VPS25 Antibody (FL-176) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-176 representing full length VPS25 of human origin |
| Modification Notes | Vacuolar sorting proteins (VPSs) are required for trafficking normal endocytic and biosynthetic proteins to the vacuole and also play an important role in the budding process of cells. VPS25 is a highly conserved and widely expressed eukaryotic protein, with single orthologs in chromalveolate, excavate, amoebozoan, plant, fungal and metazoan species. VPS25, along with Vps22p and Vps36p, functions as a subunit of ESCRT-II, an endosomal sorting complex required for protein transport. This complex is essential for the sorting of ubiquitinated biosynthetic and endosomal proteins into endosomes. ESCRT-II transiently associates with the endosomal membrane, thereby initiating the formation of ESCRT-III, a membrane-associated protein complex that functions immediately downstream of ESCRT-II during sorting of mulitvesicular body cargo. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of VPS25 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-176 representing full length VPS25 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-176 representing full length VPS25 of human origin |
| Entrez Gene | 84313 |
|---|
Application
Western blot analysis of VPS25 expression in K-562 (A) and NIH/3T3 (B) whole cell lysates.| Application Notes | recommended for detection of VPS25 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
